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Literature summary extracted from

  • Serina, L.; Bucurenci, N.; Gilles, A.M.; Surewicz, W.K.; Fabian, H.; Mantsch, H.H.; Takahashi, M.; Petrescu, I.; Batelier, G.; Barzu, O.
    Structural properties of UMP-kinase from Escherichia coli: modulation of protein solubility by pH and UTP (1996), Biochemistry, 35, 7003-7011.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 D159N appears at neutral pH almost exclusively as a hexamer Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 UTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.043
-
 UMP wild-type, at pH 7.4 Escherichia coli
2.7.4.22 0.048
-
 ATP wild-type, at pH 6.0 Escherichia coli
2.7.4.22 0.052
-
 UMP mutant D159N, at pH 7.4 Escherichia coli
2.7.4.22 0.095
-
 ATP mutant D159N, at pH 6.0 Escherichia coli
2.7.4.22 0.12
-
 ATP wild-type, at pH 7.4 Escherichia coli
2.7.4.22 0.17
-
 UMP wild-type, at pH 6.0 Escherichia coli
2.7.4.22 0.27
-
 UMP mutant D159N, at pH 6.0 Escherichia coli
2.7.4.22 0.286
-
 ATP mutant D159N, at pH 7.4 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 25800
-
 monomer, SDS-PAGE Escherichia coli
2.7.4.22 32100
-
 mutant D159N, monomer, sedimentation velocity at pH 9.0 Escherichia coli
2.7.4.22 32800
-
 wild-type, monomer, sedimentation velocity at pH 9.0 Escherichia coli
2.7.4.22 35300
-
 mutant D159N, monomer, sedimentation velocity at pH 7.4 Escherichia coli
2.7.4.22 59400
-
 wild-type, dimer, sedimentation velocity at pH 6.0 Escherichia coli
2.7.4.22 145300
-
 wild-type, hexamer, sedimentation velocity at pH 9.0 Escherichia coli
2.7.4.22 146400
-
 mutant D159N, hexamer, sedimentation velocity at pH 9.0 Escherichia coli
2.7.4.22 147400
-
 mutant D159N, hexamer, sedimentation velocity at pH 7.4 Escherichia coli
2.7.4.22 150000
-
 gel filtration Escherichia coli
2.7.4.22 157000
-
 wild-type, hexamer, sedimentation velocity at pH 6.0 Escherichia coli
2.7.4.22 297400
-
 wild-type, oligomer, sedimentation velocity at pH 9.0 Escherichia coli
2.7.4.22 482900
-
 wild-type, oligomer, sedimentation velocity at pH 6.0 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22 wild-type and mutant D159N Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 105
-
 wild-type, at pH 6.0 Escherichia coli
2.7.4.22 128
-
 mutant D159N, at pH 6.0 Escherichia coli
2.7.4.22 128
-
 wild-type, at pH 7.4 Escherichia coli
2.7.4.22 153
-
 mutant D159N, at pH 7.4 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP 30°C, pH 7.4, 5 mM MgCl2 Escherichia coli ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer alpha6, gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP-kinase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 61.6
-
 Tm Escherichia coli
2.7.4.22 62.7
-
 Tm in the presence of 0.1 mM GTP Escherichia coli
2.7.4.22 63.7
-
 Tm in the presence of 1 mM ATP Escherichia coli
2.7.4.22 65.8
-
 Tm in the presence of 1 mM GTP Escherichia coli
2.7.4.22 69.8
-
 Tm in the presence of 5 mM GTP Escherichia coli
2.7.4.22 74.6
-
 Tm in the presence of 0.1 mM UTP Escherichia coli
2.7.4.22 75.6
-
 Tm in the presence of 1 mM UMP Escherichia coli
2.7.4.22 82.4
-
 Tm in the presence of 1 mM UTP Escherichia coli
2.7.4.22 86.5
-
 Tm in the presence of 5 mM UTP Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.22 4 10
-
 Escherichia coli